Cholesterol Dehydrogenase from Nocardia sp.
| Cat# | NATE-0892 |
| Source | Nocardia sp. |
| Description | Cholesterol dehydrogenase is an enzyme that uses nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NAD(P)) as its cofactor in oxidizing cholesterol to form cholest-4-en-3-one. This enzyme oxidizes the hydroxyl group at the 3 position of the sterol ring to form a ketone. |
| Form | Freeze dried powder |
| Activity | 10U/mg-solid or more |
| CAS No. | 67775-34-2 |
| Isoelectric point | 4.5 |
| Unit Definition | One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C. |
| Activators | Triton X-100 |
| Storage | at -20°C |
| Preparation Instructions | For the activity assay, the enzyme is dissolved in 0.02 M phosphate buffer with 2.5% TRITON X-100. |
| Synonyms | Cholesterol Dehydrogenase; CDH |
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