Enzymes for Producing Triglyceride Kit

A triglyceride is an ester derived from glycerol and three fatty acids. Triglycerides are the main constituents of body fat in humans and other vertebrates, as well as vegetable fat. They are also present in the blood to enable the bidirectional transference of adipose fat and blood glucose from the liver and are a major component of human skin oils. Triglycerides are the most common lipid found in the body. They contain three chains of fatty acids. Triglycerides provide your body with energy, but their main function is to store energy for later use.

High triglycerides may contribute to the hardening of the arteries or the thickening of the artery walls (arteriosclerosis) — which increases the risk of stroke, heart attack, and heart disease. The measurement of triglyceride levels, in conjunction with other lipid assays, is useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia.

Triglyceride assay kit provides a simple, reproducible, and sensitive tool for assaying triglycerides in plasma, serum, cell lysates, and tissue homogenate samples. In the triglyceride assay, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product that reacts with a probe to generate color and fluorescence.

Creative Enzymes provides enzymes that can be used to produce triglyceride assay kits, including Lipoprotein Lipase (EC 3.1.1.34), Glycerol Kinase (EC 2.7.1.30), Glycerol-3-phosphate Oxidase (EC 1.1.99.5).

Lipoprotein lipase (EC 3.1.1.34, LPL) catalyzes the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins, thereby providing non-esterified fatty acids and 2-monoacylglycerol for tissue utilization. Abnormalities in LPL function have been found to be associated with a number of pathophysiological conditions, including atherosclerosis, chylomicronaemia, obesity, Alzheimer's disease, and dyslipidemia associated with diabetes, insulin resistance, and infection. Glycerol kinase (EC 2.7.1.30) is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol 3-phosphate. Glycerol-3-phosphate oxidase (EC 1.1.99.5) is an enzyme that catalyzes sn-glycerol 3-phosphate and O2 into dihydroxyacetone phosphate (DHAP) and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor.

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