Enzymes for Producing Creatine Kinase Assay Kit

Creatine Kinase (CK, EC 2.7.3.2), also known as creatine phosphokinase (CPK), is an enzyme expressed predominantly in skeletal muscle, smooth muscle, and the brain. The creatine kinase consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. Creatine kinase catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. Clinically, high CK in the blood may be an indication of damage to CK-rich tissue, such as in rhabdomyolysis, myocardial infarction, myositis and myocarditis. This means creatine kinase in blood may be elevated in a wide range of clinical conditions including the use of medication such as statins; endocrine disorders such as hypothyroidism; and skeletal muscle diseases and disorders including malignant hyperthermia, and neuroleptic malignant syndrome.

Creatine kinase activity assay kit uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity. In the creatine kinase assay, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.

Creative Enzymes provides raw materials that can be used to produce creatine kinase assay kits, including hexokinase (EC 2.7.1.1), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), creatine kinase MM (EC 2.7.3.2), and creatine kinase MB (EC 2.7.3.2).

Hexokinases are intracellular enzymes that phosphorylate glucose, mannose and fructose to the corresponding hexose 6-phosphates. The resulting phosphate esters can then be broken down to pyruvate by glycolysis or used for different biosynthesis. Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) is a cytosolic enzyme that catalyzes D-glucose 6-phosphate, NADP⁺, and H₂O into 6-phospho-D-glucono-1,5-lactone, NADPH, and H⁺. This enzyme participates in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). Clinically, an X-linked genetic deficiency of G6PD makes a human prone to non-immune hemolytic anemia. Creatine kinase MM (CK-MM) and creatine kinase MB (CK-MB) are isoenzymes of creatine kinase. CK is a dimeric molecule, composed of M and B subunits, Skeletal muscle, myocardium, and neuronal tissue are the main sources of CK-MM, CK-MB, and CK-BB, respectively.