Enzymes for Producing Free Fatty Acid Assay Kit

Triglycerides (TAG) are a type of lipid in the blood, serving as an energy source and playing a key role in metabolism. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. Free fatty acids (FFA, also monoacylglycerols and diacylglycerols) are derived from triacylglycerol by cleavage of ester bonds due to the action of lipase, high temperature, and moisture. Besides the direct oxidation, lipid hydrolysis is the dominant reason for the generation of free fatty acids when the oils were entered the second stage of lipid oxidation. Additionally, hormones induce and regulate lipase activity in adipose tissue, resulting in changes to blood FFA levels. Free fatty acids then bind plasma albumin for circulation in the body, serving as a readily absorbed energy source for muscle, brain and other organ tissues. Measurement of free fatty acids has become useful in monitoring and diagnosis of several diseases and metabolic disorders (e.g. obesity, insulin resistance, diabetes, cancer).

Free Fatty Acid Assay Kit uses a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc. In the free fatty acid assay, fatty acids are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric or fluorometric methods.

Creative Enzymes provides high-quality Acyl-CoA synthetase (EC 6.2.1.3) and Acyl-CoA oxidase (EC 1.3.3.6), which are raw materials used in the production of free fatty acid assay kits.

Fatty acyl-CoA synthetase (ACS) catalyzes the adenosine triphosphate dependent formation of a thioester bond between a fatty acid and coenzyme A. This fundamental reaction allows the fatty acid to be degraded for energy production, incorporated into complex lipids, or participate in other metabolic pathways. The role of the ACS enzyme is to combine acetate and CoA to form acetyl CoA, however its significance is much larger. The most well-known function of the product from this enzymatic reaction is the use of Acetyl-CoA in the role of the TCA cycle as well as in the production of fatty acid. This enzyme is vital to the action of histone acetylation as well as gene regulation. It catalyzes acyl-CoA and O2 into trans-2,3-dehydroacyl-CoA and H2O2. This enzyme participates in 3 metabolic pathways: fatty acid metabolism, polyunsaturated fatty acid biosynthesis, and PPAR signaling pathway.