Glycerol-3-phosphate oxidase from Microorganism
| Cat# | DIA-200 |
| Abbr | L-α-glycerophosphate oxidase (Microorganism) |
| Source | Microorganism |
| Description | In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction: sn-glycerol 3-phosphate + O2 ↔ glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD. |
| Applications | This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis. |
| Appearance | Yellowish amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.3.21 |
| Activity | 15 U/mg-solid or more (containing approx. 60% of stabilizers) |
| CAS No. | 9046-28-0 |
| Contaminants | Lactate oxidase < 2.0×10⁻⁴%| Phosphatase < 1.0×10⁻³%| |
| Isoelectric point | 4.6±0.1 |
| pH Stability | 5.0-7.5 (25°C, 60min) |
| Michaelis Constant | 2.3×10⁻³M (L-α-Glycerophosphate) |
| Optimum pH | 6.5-7.0 |
| Optimum temperature | 40°C |
| Thermal stability | below 45°C (pH 6.5, 10min) |
| Stability | Stable at-20°C for at least 6 months |
| Stabilizers | Sucrose, FAD |
| Inhibitors | SH-reagents, ionic detergents, metal ions, etc. |
| Synonyms | L-α-glycerophosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; EC 1.1.3.21 |
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