| Cat# | DIA-211 |
| Source | Microorganism |
| Description | Lipoprotein lipase (LPL) (EC 3.1.1.34) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires ApoC-II as a cofactor. |
| Activity | GradeⅢ 1.0U/mg-solid or more |
| CAS No. | 9004-02-8 |
| Synonyms | Lipoprotein lipase; LPL; EC 3.1.1.34; Clearing factor lipase; Diacylglycerol lipase; Diglyceride lipase |
| Enzyme Commission Number | EC 3.1.1.34 |
| pH Stability | pH 5.5-10.0 (25°C, 20hr) |
| Optimum pH | 8 |
| Optimum temperature | 40-45°C |
| Thermal stability | below 40°C (pH 7.0, 15min) |
| Stability | Store at -20°C |
| Stabilizers | Mg??, bovine serum albumin |
| Inhibitors | Hg??, Cu?? |
| Contaminants | Phosphatase 1.0×10?3% Catalase 2.0×10?2% NADH oxidase1.0×10?3% Cholesterol oxidase 2.0×10?3% |
| Abbr | LPL (Microorganism) |
| Alias | LPL |
| Applications | This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0. |
| Appearance | Light brown amorphous powder, lyophilized |
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