Native Microorganism Malate Dehydrogenase
| Cat# | DIA-160 |
| Abbr | MDH (Microorganism) |
| Alias | NAD-sp; MDH |
| Source | Microorganism |
| Description | Malate dehydrogenase is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of malate to pyruvate producing NADPH. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate. |
| Applications | This enzyme is useful for enzymatic determination of L-malate and of glutamate oxaloacetate transaminase (GOT) in clinical analysis. |
| Appearance | Slightly yellowish amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.1.37 |
| Activity | 40U/mg-solid or more |
| CAS No. | 9001-64-3 |
| Contaminants | Glutamate oxaloacetate transaminase < 1.0×10⁻³% Lactate dehydrogenase < 1.0×10⁻³% NADH oxidase< 1.0×10⁻³% |
| Isoelectric point | pH 4.8±0.1 |
| pH Stability | pH 3.0-9.0 (25°C, 20hr) |
| Michaelis Constant | 5.4×10⁻⁵M (L-Malate), 5.0×10⁻⁶M (Oxaloacetate), 8.1×10⁻⁶M (NADH) |
| Structure | 4 subunits per mole of enzyme |
| Optimum pH | 8 |
| Optimum temperature | 70°C |
| Thermal stability | below 70°C (pH 7.5, 15min) |
| Storage | Stable at-20°C for at least one year |
| Inhibitors | Hg⁺⁺ |
| Synonyms | malic dehydrogenase; L-malate dehydrogenase; NAD-L-malate dehydrogenase; malic acid dehydrogenase; NAD-dependent malic dehydrogenase; NAD-malate dehydrogenase; NAD-malic dehydrogenase; malate NAD dehydrogenase; NAD-dependent malate dehydrogenase; NAD-sp; ECific malate dehydrogenase; NAD-linked malate dehydrogenase; MDH; L-malate-NAD+ oxidoreductase; S-malate: NAD+ oxidoreductase; EC 1.1.1.37; Malate Dehydrogenase |
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