| Cat# | NATE-0059 |
| Abbr | ALP, Native (Porcine) |
| Alias | ALP; ALKP |
| Species | Porcine |
| Source | Porcine kidney |
| Description | Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. |
| Applications | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling. |
| Package | Package size based on DEA units |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 3.1.3.1 |
| Activity | >100U/mg |
| CAS No. | 9001-78-9 |
| Unit Definition | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37°C. |
| Storage | −20°C |
| Warnings | Protein determined by biuret. |
| Synonyms | Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase |
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