Native Proteus sp. Glutamate Dehydrogenase (NADP-dependent)
| Cat# | DIA-196 |
| Abbr | GLDH (NADP) (Proteus sp.) |
| Alias | GLDH |
| Source | Proteus sp. |
| Description | Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate. |
| Applications | This enzyme is useful for enzymatic determination of NH₃, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis. |
| Appearance | Solution with 50mM Tris-HCl buffer containing 0.05% NaN₃ and 5.0mM EDTA, pH 7.8 |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.4.1.4 |
| Activity | 300U/mg-protein or more (9,000U/ml or more) |
| CAS No. | 2604121 |
| Contaminants | NADPH oxidase < 1.0×10⁻²% Glutathione reductase < 1.0×10⁻²% (GradeⅡ-209) < 1.0×10⁻¹% (GradeⅢ-309) |
| Molecular Weight | approx. 300 kDa |
| Isoelectric point | 4.6 |
| pH Stability | pH 6.0-8.5 (25°C, 20hr) |
| Michaelis Constant | 1.1×10⁻³M (NH₃), 3.4×10⁻⁴M (α-Ketoglutarate), 1.2×10⁻³M (L-Glutamate), 1.4×10⁻⁵M (NADPH), 1.5×10⁻⁵M (NADP⁺) |
| Structure | 6 subunits (M.W.50,000) per mol of enzyme |
| Optimum pH | 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG) |
| Optimum temperature | 45°C (α-KG→L-Glu) 45-55°C (L-Glu→α-KG) |
| Thermal stability | below 50°C (pH 7.4, 10min) |
| Stability | Stable at 5°C for at least 6 months |
| Stabilizers | Ethylenediaminetetraacetic acid (EDTA) |
| Inhibitors | Hg⁺⁺, Cd⁺⁺, p-chloromercuribenzoate, pyridine, 4-4'-dithiopyridine, 2,2'-dithiopyridine |
| Synonyms | glutamate dehydrogenase (NADP+); glutamic dehydrogenase; dehydrogenase; glutamate (nicotinamide adenine dinucleotide (phosphate)); glutamic acid dehydrogenase; L-glutamate dehydrogenase; L-glutamic acid dehydrogenase; NAD(P)-glutamate dehydrogenase; NAD(P)H-dependent glutamate dehydrogenase; glutamate dehydrogenase (NADP); EC 1.4.1.4; GLDH |
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