Pyranose Oxidase from Microorganism
| Cat# | NATE-1718 |
| Abbr | PROD (Microorganism) |
| Source | Microorganism |
| Description | Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. P2O is a homotetrameric protein that contains covalently bound flavin adenine dinucleotide (FAD). The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide. |
| Form | Yellow power, lyophilized |
| Enzyme Commission Number | EC 1.1.3.10 |
| Activity | >10U/mg protein |
| CAS No. | 37250-80-9 |
| Molecular Weight | 70kDa (SDS-PAGE) |
| Isoelectric point | 5.95 |
| pH Stability | 4.0~11.0(50℃,30min) |
| Michaelis Constant |
8.6 10^-3 M(1.5 anlydroglucitol) 7.12×10^-4 M (D-glucose ) |
| Unit Definition | One unit will convert one micromole of Dglucose to 2-Dehydro-D-glucose per minute at pH 7.0 at 37℃. |
| Optimum pH | 6 |
| Optimum temperature | 60℃ |
| Thermal stability | <55℃ (pH 7.0, 30min) |
| Storage | Store at -20°C. |
| Inhibitors | Ag+, Hg2+ |
| Synonyms | EC 1.1.3.10; glucose 2-oxidase; pyranose-2-oxidase; 37250-80-9; P2O |
Download Datasheet:
|
