Native Microorganism Glucose-6-phosphate Dehydrogenase
| Cat# | DIA-145 |
| Abbr | G6PDH (Microorganism) |
| Alias | G6PDH; GPD; G6PD1; G6PD |
| Source | Microorganism |
| Description | Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). |
| Applications | The enzyme is useful for enzymatic determination of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase. |
| Appearance | White amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.1.49 |
| Activity | 200U/mg-solid or more |
| CAS No. | 9001-40-5 |
| Contaminants | Creatine phosphokinase < 1×10⁻³% Phosphoglucomutase < 1×10⁻³% 6-Phosphogluconate dehydrogenase < 5×10⁻³% Phosphoglucose isomerase < 1×10⁻²% Glutathione reductase < 1×10⁻³% Hexokinase < 1×10⁻²% Myokinase < 1×10⁻²% NADH oxidase < 1×10⁻²% NADPH oxidase < 1×10⁻²% |
| Molecular Weight | approx. 140 kDa (by gel filtration) |
| pH Stability | pH 5.0-11.0 (25°C, 22hr) |
| Michaelis Constant | NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P), NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P) |
| Optimum pH | 7.8 |
| Optimum temperature | 50°C-55°C |
| Thermal stability | below 50°C (pH 7.8, 30min) |
| Stability | Stable at-20°C for at least one year |
| Inhibitors | Metal ions, iodoacetamimide, SDS etc. |
| Synonyms | Glucose-6-phosphate dehydrogenase; G6PD; G6PDH; Glucose-6-phosphate dehydrogenase (NADP(+)); EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate dehydrogenase; GPD |
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