diagnostic-enzymes

Native Microorganism Glucose-6-phosphate Dehydrogenase

Native Microorganism Glucose-6-phosphate Dehydrogenase

Cat# AbbrAliasSimilarSourceDescriptionApplicationsAppearanceFormEnzyme Commission NumberActivityCAS No. ContaminantsMolecular WeightpH StabilityMichaelis ConstantOptimum pHOptimum temperatureThermal stabilityStabilityInhibitorsSynonyms
DIA-145 G6PDH (Microorganism)G6PDH; GPD; G6PD1; G6PDGlucose-6-phosphate dehydrogenaseMicroorganismGlucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).The enzyme is useful for enzymatic determination of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.White amorphous powder, lyophilizedFreeze dried powderEC 1.1.1.49200U/mg-solid or more9001-40-5Creatine phosphokinase < 1×10⁻³% Phosphoglucomutase < 1×10⁻³% 6-Phosphogluconate dehydrogenase < 5×10⁻³% Phosphoglucose isomerase < 1×10⁻²% Glutathione reductase < 1×10⁻³% Hexokinase < 1×10⁻²% Myokinase < 1×10⁻²% NADH oxidase < 1×10⁻²% NADPH oxidase < 1×10⁻²%approx. 140 kDa (by gel filtration)pH 5.0-11.0 (25°C, 22hr)NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P), NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P)7.850°C-55°Cbelow 50°C (pH 7.8, 30min)Stable at-20°C for at least one yearMetal ions, iodoacetamimide, SDS etc.Glucose-6-phosphate dehydrogenase; G6PD; G6PDH; Glucose-6-phosphate dehydrogenase (NADP(+)); EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate dehydrogenase; GPD Download Datasheet:
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