diagnostic-enzymes

Native Microorganism Hexokinase

Native Microorganism Hexokinase

Cat# AbbrAliasSimilarSourceDescriptionApplicationsAppearanceFormEnzyme Commission NumberActivityContaminantsMolecular WeightIsoelectric pointpH StabilityMichaelis ConstantOptimum pHOptimum temperatureThermal stabilityStabilityInhibitorsSynonyms
DIA-202 Hexokinase (Microorganism)HexokinaseHexokinaseMicroorganismA hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.The enzyme is useful for enzymatic determination of glucose, adenosine-5'-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase.White amorphous powder, lyophilizedFreeze dried powderEC 2.7.1.1150U/mg-solid or morePhosphoglucose isomerase < 1.0×10⁻¹% 6-Phosphogluconate dehydrogenase < 1.0×10⁻²% Glucose-6-phosphate dehydrogenase < 1.0×10⁻²% Myokinase < 1.0×10⁻²% Glutathione reductase < 5.0×10⁻¹%approx. 82 kDa (by gel filtration)4.1±0.1pH 4.0-9.0 (25°C, 20hr)2.3×10⁻⁴M (D-Glucose), 7.7×10⁻⁵M (ATP)8.0-9.050°Cbelow 45°C (pH 7.0, 30min)Store at-20°Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etcHexokinase; EC 2.7.1.1; hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; ATP: D-hexose 6-phosphotransferase Download Datasheet:
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