| Cat# | DIA-202 |
| Abbr | Hexokinase (Microorganism) |
| Alias | Hexokinase |
| Source | Microorganism |
| Description | A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. |
| Applications | The enzyme is useful for enzymatic determination of glucose, adenosine-5'-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase. |
| Appearance | White amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 2.7.1.1 |
| Activity | 150U/mg-solid or more |
| Contaminants | Phosphoglucose isomerase < 1.0×10⁻¹% 6-Phosphogluconate dehydrogenase < 1.0×10⁻²% Glucose-6-phosphate dehydrogenase < 1.0×10⁻²% Myokinase < 1.0×10⁻²% Glutathione reductase < 5.0×10⁻¹% |
| Molecular Weight | approx. 82 kDa (by gel filtration) |
| Isoelectric point | 4.1±0.1 |
| pH Stability | pH 4.0-9.0 (25°C, 20hr) |
| Michaelis Constant | 2.3×10⁻⁴M (D-Glucose), 7.7×10⁻⁵M (ATP) |
| Optimum pH | 8.0-9.0 |
| Optimum temperature | 50°C |
| Thermal stability | below 45°C (pH 7.0, 30min) |
| Stability | Store at-20° |
| Inhibitors | Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etc |
| Synonyms | Hexokinase; EC 2.7.1.1; hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; ATP: D-hexose 6-phosphotransferase |
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