Creatinase from E. coli, Recombinant
Creatinase from E. coli, Recombinant

Creatinase from E. coli, Recombinant

Cat# NATE-1241
Abbr Creatinase, Recombinant (E. coli)
Species E. coli
Source E. coli
Description In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Appearance White lyophilizate
Enzyme Commission Number EC 3.5.3.3
Activity > 15 U/mg
CAS No. 37340-58-2
Contaminants catalase < 0.5%
Molecular Weight ca. 80 kDa
pH Stability 4.0–11.0
Michaelis Constant 8.6 x 10^-3 M (creatine)
Structure 2 subunits of 48 kDa (SDS-PAGE)
Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of urea per min at 37°C and pH 7.7.
Optimum pH 7.0–9.0
Optimum temperature 45°C
Thermal stability below 53°C
Storage at -20°C
Stabilizers Sucrose
Inhibitors Hg2+
Synonyms Creatine amidohydrolase; Creatinase; EC 3.5.3.3
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