D-3-hydroxybutyrate dehydrogenase from Microorganism
D-3-hydroxybutyrate dehydrogenase from Microorganism

D-3-hydroxybutyrate dehydrogenase from Microorganism

Cat# NATE-1714
Abbr HBDH (Microorganism)
Source Microorganism
Description In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.
Form Yellowish powder, lyophilized
Enzyme Commission Number EC 1.1.1.30
Activity >300U/mg
CAS No. 9028-38-0
Molecular Weight 27.5 kDa (SDS-PAGE)
Isoelectric point 7.25
pH Stability 7.0~10.0 (25℃, 2 hr)
Michaelis Constant 2.1 ×10^-3 M (D-3-Hydroxybutyrate)
Unit Definition One unit converts one micromole of 3- Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
Optimum pH 8
Optimum temperature 6 ℃
Thermal stability < 37℃(pH 8.5, 30min)
Storage Store at -20°C.
Inhibitors Zn2+, Cu2+, Fe3+
Synonyms (R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30
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