| Cat# |
NATE-1714 |
| Abbr |
HBDH (Microorganism) |
| Source |
Microorganism |
| Description |
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism. |
| Form |
Yellowish powder, lyophilized |
| Enzyme Commission Number |
EC 1.1.1.30 |
| Activity |
>300U/mg |
| CAS No. |
9028-38-0 |
| Molecular Weight |
27.5 kDa (SDS-PAGE) |
| Isoelectric point |
7.25 |
| pH Stability |
7.0~10.0 (25℃, 2 hr) |
| Michaelis Constant |
2.1 ×10^-3 M (D-3-Hydroxybutyrate) |
| Unit Definition |
One unit converts one micromole of 3- Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
|
| Optimum pH |
8 |
| Optimum temperature |
6 ℃ |
| Thermal stability |
< 37℃(pH 8.5, 30min) |
| Storage |
Store at -20°C. |
| Inhibitors |
Zn2+, Cu2+, Fe3+ |
| Synonyms |
(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30 |
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