Native Jack bean Urease
Native Jack bean Urease

Native Jack bean Urease

Cat# PHAM-180
Abbr Urease (Jack bean)
Alias Urease
Source Jack bean
Description Ureases (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O → CO2 + 2NH3.
Applications This enzyme is useful for enzymatic determination of urea in clinical analysis.
Appearance White amorphous powder, lyophilized
Form Freeze dried powder
Enzyme Commission Number EC 3.5.1.5
Activity 100U/mg-solid or more
CAS No. 9002-13-5
Contaminants Asparaginase < 2.0×10⁻²% Arginase < 2.0×10⁻³% NH₄⁺ < 5.0×10⁻⁴µg/U
Isoelectric point 5.0-5.1
pH Stability pH 5.5-8.5 (30°C, 17hr)
Michaelis Constant 1.05×10⁻²M (Urea)
Structure 8 active sites with SH-groups per mole of the enzyme
Optimum pH 6
Optimum temperature 60°C
Thermal stability below 50°C (pH 8.0, 60min)
Stability Store at -20°C (A decrease in activity of ca.15% may occur within 6 months)
Stabilizers EDTA, glutathione, succinate, BSA
Inhibitors Heavy metal ions (Ag⁺,Hg⁺⁺,etc.)
Synonyms EC 3.5.1.5; Urease
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