| Cat# | BDE-010 |
| Description | Malate dehydrogenase (MDH), widely distributed across species, catalyzes the reversible conversion between oxaloacetate and malate. In serum CO₂ detection, phosphoenolpyruvate carboxylase (PEPC) converts bicarbonate and phosphoenolpyruvate (PEP) to oxaloacetate, which is then reduced to malate by MDH, coupling NADH oxidation to NAD⁺. |
| Applications | Used in coupled assays for oxaloacetate or malate; it is a commonly used auxiliary enzyme in liver function and cardiac enzyme panel kits. |
| CAS No. | 9001-64-3 |
| Enzyme Commission Number | EC 1.1.1.37 |
| Form | White amorphous powder, lyophilized |
| Source | Microorganism |
| Activity | ≥ 300 U/mg-solid; ≥ 900 U/mg-protein |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the oxidation of one micromole of NADH per min at pH 7.5 at 37°C. |
| Molecular Weight | 35 kDa (SDS-PAGE) |
| Optimum pH | 7.5-8.0 |
| Optimum temperature | 60°C |
| Storage | Aliquot and store at ≤ -20 °C. |
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