| Cat# | DIA-542 |
| Source | Escherichia coli |
| Description | High purity phosphoglucose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 5.3.1.9 |
| Activity | ~ 550 U/mg of protein (25 °C, pH 7.6 on fructose 6-phosphate) ~ 649 U/mg of protein (40 °C, pH 7.6 on fructose 6-phosphate) |
| CAS No. | 9001-41-6 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.6. |
| Storage | 2–8 °C |
| Synonyms | Glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
| Molecular Weight | 62600 Da |
| Concentration | 10 KU: ~ 1,000 U/mL 50 KU: ~ 10,000 U/mL |
| Package | 10/50 KU |
| Specificity | Catalyses the reaction: D-Glucose 6-phosphate = D-fructose 6-phosphate. |
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