| Cat# |
DIA-536 |
| Source |
Bacillus licheniformis |
| Description |
High purity protease (subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form |
Solution |
| ECNumber |
3.4.21.62 |
| Activity |
~ 6 U/mg of protein (40 °C, pH 8.0 on casein) |
| CAS No. |
9014-01-1 |
| Optimum temperature |
60 °C |
| Stability |
> 1 year under recommended storage conditions |
| Unit Definition |
One unit will hydrolyse casein to produce colour equivalent to one µmole (181 µg) of tyrosine per minute at pH 7.0 at 40 °C. |
| Storage |
2–8 °C |
| Synonyms |
Subtilisin; subtilisin A |
| Buffer |
50% (v/v) glycerol |
| Molecular Weight |
30250 Da |
| Specificity |
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
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