| Cat# | ENZD-P04 |
| Specification | 250 U; 1000 U; 3000 U |
| Description | AdvTaq DNA Polymerase is a chemically modified Taq DNA Polymerase whose activity is completely blocked at room temperature, preventing non-specific amplification and primer dimers during sample preparation and reaction heating. Its activity is only released after heating to 95°C. Compared to antibody-based hot-start Taq enzymes, AdvTaq DNA Polymerase offers more thorough and stringent activity blocking; compared to existing chemically modified hot-start Taq enzymes, AdvTaq DNA Polymerase requires only 5 minutes of activation time and is compatible with existing PCR programs. AdvTaq DNA Polymerase, combined with an optimized buffer system, significantly reduces non-specific amplification and primer dimers, resulting in high sensitivity, making it ideal for amplifying low-copy genes from complex templates (genomic DNA, cDNA). The PCR product has an A-terminus at the 3' end, allowing cloning into T-vectors. |
| Features | Highly rigorous: Utilizing chemical modification, enzyme activity is completely blocked below 80℃. Rapid activation: Heating at 95℃ for 5 minutes releases the active ingredient. High sensitivity: Suitable for amplifying low-copy genes from complex templates (genome, cDNA). Easy to use: Provides ready-to-use premixed solutions, greatly reducing experimental steps. |
| Applications | Colony PCR |
| Components | 10 x AdvTaq Buffer (Mg²⁺ Plus) dNTP Mix (10 mM each) AdvTaq DNA Polymerase (5 U/µl) |
| Shipping and Storage Conditions | Store at -20℃ |
| Download Datasheet: |  |
ENZD-P04 was evaluated for amplification sensitivity using serial dilutions of plasmid DNA ranging from 10-2 ng to 10-7 ng. The performance was compared with a commercial enzyme from Supplier T under identical qPCR conditions. Across equivalent template concentrations, ENZD-P04 consistently demonstrated higher sensitivity, enabling more efficient detection of low-abundance targets. This performance highlights its reliability as a high-quality enzyme for applications requiring precise and sensitive nucleic acid detection. Data generated from internal qPCR performance evaluation.
