| Cat# | CAS-1302 |
| Source | Recombinant |
| Description | LbuCas13a (also known as C2c2) originates from the strain Leptotrichia buccalis. LbuCas13a is a type II VI CRISPR system effector protein, a crRNA-mediated endonuclease. Its "accessory cleavage" activity is activated when recognizing and cleaving target RNA, allowing it to non-specifically cleave single-stranded RNA (ssRNA) in the system. By designing RNA probes with fluorescent groups or other labels at both ends, CRISPR/Cas13a can be used to detect and amplify the RNA template. Results can be observed using a fluorometer or test strips. |
| Storage | Shelf life is 1 year. Storage conditions: -80°C. To avoid repeated freeze-thaw cycles, after opening, please store the Cas enzyme at -20°C. It is recommended to aliquot according to the number of uses to avoid repeated temperature changes that may reduce enzyme activity. |
| Purity | 95% (SDS-PAGE) |
| Optimum temperature | 37°C |
| Abbr | LbuCas13a |
| Applications | RNA Detection; Diagnostics |
| Appearance | Liquid |
| Molecular Weight | 140.6 kDa |
| Species | Leptotrichia buccalis |
| Grade | Research Grade |
| reaction system | 1 μM LbuCas13a, 5 × Cleavage Buffer, 25 U RNase Inhibitor, 500 nM crRNA, 4 μM ssRNA Reporter, 1 μM RNA target, DEPC H2O |
| Download Datasheet: |  |
