| Cat# | DIA-515 |
| Source | Escherichia coli |
| Description | High purity recombinant β-glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Solution |
| ECNumber | 3.2.1.31 |
| Activity | ~ 15,000 U/mg (37 °C, pH 6.8 on phenolphthalein-β-D-glucuronide) ~ 50 U/mg (37 °C, pH 7.5 on pNP-β-D-glucuronide) |
| Optimum pH | 6.8 |
| Optimum temperature | 37 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | 30000 U/mg protein: One unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37 °C. 110 U/mg protein: One unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris HCl buffer (100 mM) pH 7.5 and 37 °C, monitored at 410 nm. |
| Storage | 2–8 °C |
| Synonyms | β-D-glucuronoside glucuronosohydrolase; GUS |
| Buffer | Tris HCl/NaCl/EDTA |
| Molecular Weight | 82600 Da |
| Concentration | ~ 250 U/mL |
| Specificity | Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates. |
| Download Datasheet: |  |
