| Cat# |
DIA-518 |
| Source |
Clostridium thermocellum |
| Description |
High purity chitinase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form |
Suspension |
| Activity |
~ 0.3 U/mg (40 °C, pH 6.2 on p-nitrophenyl-β-triacetylchitotriose) |
| CAS No. |
9001-06-3 |
| Optimum temperature |
40 °C |
| Stability |
> 1 year under recommended storage conditions |
| Unit Definition |
One unit of chitinase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from p-nitrophenyl-β-triacetylchitotriose (2.5 mM) in MES buffer (100 mM), pH 6.2 at 40 °C. |
| Storage |
2–8 °C |
| Synonyms |
Chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase |
| Buffer |
3.2 M ammonium sulphate |
| Molecular Weight |
53000 Da |
| Concentration |
~ 3 U/mL |
| Specificity |
Random hydrolysis of N-acetyl-beta-D-glucosaminide (1-4)-beta-linkages in chitin and chitodextrins. |
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