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Chitinase from Clostridium thermocellum

Cat# DIA-518
Source Clostridium thermocellum
Description High purity chitinase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form Suspension
Activity ~ 0.3 U/mg (40 °C, pH 6.2 on p-nitrophenyl-β-triacetylchitotriose)
CAS No. 9001-06-3
Optimum temperature 40 °C
Stability > 1 year under recommended storage conditions
Unit Definition One unit of chitinase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from p-nitrophenyl-β-triacetylchitotriose (2.5 mM) in MES buffer (100 mM), pH 6.2 at 40 °C.
Storage 2–8 °C
Synonyms Chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase
Buffer 3.2 M ammonium sulphate
Molecular Weight 53000 Da
Concentration ~ 3 U/mL
Specificity Random hydrolysis of N-acetyl-beta-D-glucosaminide (1-4)-beta-linkages in chitin and chitodextrins.
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