| Cat# | DIA-546 |
| Source | Candida boidinii |
| Description | High purity recombinant formate dehydrogenase from Candida boidinii for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| CAS No. | 9028-85-7 |
| Activity | ~ 2 U/mg (25 °C, pH 7.6 on formic acid) |
| Unit Definition | One unit of formate dehydrogenase is defined as the amount of enzyme required to convert one μmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Formate dehydrogenase; formate:NAD+ oxidoreductase |
| Enzyme Commission Number | EC 1.2.1.2 (→ EC 1.17.1.9) |
| Stability | > 1 year under recommended storage conditions |
| Optimum pH | 7.6 |
| Optimum temperature | 37 °C |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries. |
| Molecular Weight | 41331 Da |
| Concentration | ~ 75 U/mL |
| Specificity | Catalyses the reaction: Formate + NAD+ = CO2+ + NADH. |
| Download Datasheet: |  |
