| Cat# | DIA-521 |
| Source | Aspergillus niger |
| Description | High purity endo-1,4 β-mannanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.2.1.78 |
| Activity | ~ 50 U/mg (40 °C, pH 4.0 on carob galactomannan) |
| CAS No. | 37288-54-3 |
| Optimum temperature | 60 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Mannan endo-1,4-β-mannosidase; 4-β-D-mannan mannanohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications established in analysis and research within the food and feed, carbohydrate, biofuels and paper production industries. |
| Molecular Weight | 48000 Da |
| Concentration | ~ 600 U/mL |
| Specificity | Random hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans. |
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