| Cat# | DIA-459 |
| Description | Antibody-modified Tth DNA Polymerase. Antibody blocks polymerase activity at low temperatures, preventing non-specific amplification. More resistant to blood-derived inhibitors than Taq, with enhanced reverse transcription activity in the presence of Mn2+. Suitable for one-step RT-PCR. Glycerol-free, compatible with lyophilized formulations. |
| Storage | –20 °C |
| Contaminants | Free of E. coli DNA residues; free of endo-/exonuclease contamination |
| Product Overview | Product Components: 250 U: HotStart Tth DNA Polymerase (Glycerol-free, 5U/μL): 50 μL 10x PCR buffer: 500 μL 5x RT-PCR buffer (Mn2+ Free): 1 mL MnCl2 (25mM): 300 μL 2500 U: HotStart Tth DNA Polymerase (Glycerol-free, 5U/μL): 500 μL 10x PCR buffer: 5 mL 5x RT-PCR buffer (Mn2+ Free): 10 mL MnCl2 (25mM): 1 mL × 3 Product Features: Dual enzyme activity: combines reverse transcriptase and polymerase for one-step detection Higher RT temperature (60–70 °C): ensures superior reverse transcription specificity Strong resistance to PCR inhibitors |
| Package | 250/2500 U |
| Download Datasheet: |  |
