| Cat# | DIA-525 |
| Source | Aspergillus niger |
| Description | High purity recombinant exo-inulinase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.2.1.80 |
| Activity | ~ 1,600 U/mg (60 °C, pH 4.5 on kestose) ~ 800 U/mg (40 °C, pH 4.5 on kestose) |
| CAS No. | 37288-56-5, 9001-57-4 |
| Optimum temperature | 60 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Fructan β-fructosidase; β-D-fructan fructohydrolase; β-fructofuranosidase; β-D-fructofuranoside fructohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications established in food industry for fructose syrup production and in the analysis industry for the measurement of fructans and inulins. |
| Molecular Weight | 58400 Da |
| Concentration | ~ 2,000 U/mL (40 °C, pH 4.5 on kestose) |
| Specificity | EC 3.2.1.80: Hydrolysis of terminal, non-reducing (2,1)- and (2,6)-linked β-D-fructofuranose residues in fructans. EC 3.2.1.26: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
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