| Cat# | DIA-526 |
| Source | Rumen microorganism |
| Description | High purity recombinant feruloyl esterase (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.1.1.73 |
| Activity | ~ 30 U/mg (40 °C, pH 6.5 on ethyl ferulate) |
| CAS No. | 134712-49-5, 224306-54-1, 224306-55-2 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of feruloyl esterase activity is defined as the amount of enzyme required to release one μmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries. |
| Molecular Weight | 29000 Da |
| Concentration | ~ 400 U/mL |
| Specificity | Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. |
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