| Cat# | DIA-546 |
| Source | Candida boidinii |
| Description | High purity recombinant formate dehydrogenase (Candida boidinii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 1.2.1.2 (transferred to EC 1.17.1.9) |
| Activity | ~ 2 U/mg (25 °C, pH 7.6 on formic acid) |
| CAS No. | 9028-85-7 |
| Optimum pH | 7.6 |
| Optimum temperature | 37 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of formate dehydrogenase is defined as the amount of enzyme required to convert one μmole of formic acid to NADH and CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Formate:NAD+ oxidoreductase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries. |
| Molecular Weight | 41331 Da |
| Concentration | ~ 75 U/mL |
| Specificity | Catalyses the reaction: Formate + NAD+ = CO2+ + NADH. |
| Download Datasheet: |  |
