| Cat# | DIA-547 |
| Source | Escherichia coli |
| Description | High purity galactose dehydrogenase (Escherichia coli) and galactose mutarotase (Escherichia coli) mixtrure for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | Galactose dehydrogenase: 1.1.1.48 Galactose mutarotase: 5.1.3.3 |
| Activity | Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25 °C Galactose mutarotase: 4.1 mg/mL |
| CAS No. | Galactose dehydrogenase: 9028-54-0 Galactose mutarotase: 9031-76-9 |
| Optimum pH | 8.6 |
| Optimum temperature | 25 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at pH 8.6, at 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR). |
| Specificity | Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). (1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+. |
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