| Cat# | DIA-584 |
| Source | Escherichia coli |
| Description | T4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 5′-phosphate and 3′-hydroxyl termini in double-stranded DNA or RNA. It efficiently ligates both blunt-ended and cohesive-ended fragments and can also repair single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrid duplexes. |
| Unit Definition | One unit is defined as the amount of enzyme required to ligate 50% of HindIII-digested λDNA fragments (100 ng) in a 50 μL reaction containing 1× T4 DNA ligase buffer at 23 °C for 30 minutes. |
| Storage | -20 °C |
| Buffer | 20 mM Tris-HCl pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol |
| Applications | Catalyzes ligation between blunt or cohesive ends; repairs single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids. |
| Product Overview | Rapid ligation at room temperature: Efficient ligation of cohesive or blunt ends in 5 minutes. For T/A cloning. Repair of nicks in double-stranded DNA. |
| Package | 600000 U T4 DNA Ligase (Rapid)(600 U/μL): 1 mL 2× Rapid Ligation Buffer (132 mM Tris-HCI pH 7.6 25 °C, 20 mM MgCl2, 2 mM DTT, 2 mM ATP, 15% PEG 6000): 6 mL 10× T4 DNA Ligase Buffer (500 mM Tris-HCI pH 7.6 25 °C, 100 mM MgCl2, 50 mM DTT, 10 mM ATP): 6 mL |
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