| Cat# | DIA-538 |
| Source | Klebsiella planticola |
| Description | High purity pullulanase M1 (Klebsiella planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Solution |
| Activity | ~ 30 U/mg (40 °C, pH 5.0 on pullulan) |
| CAS No. | 9075-68-7 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Pullulanase; pullulan 6-α-glucanohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups. |
| Molecular Weight | 109000 Da |
| Concentration | ~ 650 U/mL |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen. |
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