| Cat# | Kit-068 |
| Description | Designed for Illumina sequencing, this kit includes a DNA damage repair module for FFPE samples. It converts 100 pg–1 μg input DNA into sequencing-ready libraries. The DNA repair module effectively restores FFPE-induced DNA damage, such as cytosine deamination, nicks, gaps, base oxidation, and 3′-end blocking, while maintaining compatibility with standard DNA samples. Optimizations in end-repair, ligation, and amplification modules increase library conversion rates and yield, supporting both PCR and PCR-free library construction, as well as targeted capture workflows. All reagents are thoroughly quality-controlled and functionally validated. |
| Storage | -30 °C to -15 °C |
| Applications | Whole-genome sequencing; whole-exome or other targeted capture sequencing; amplicon sequencing; metagenomic sequencing; methylation sequencing. |
| Product Overview | Efficient DNA damage repair: Effectively repairs base damage, nicks, gaps, and 3′-end blocking lesions. High library construction efficiency: Improved library conversion efficiency and amplification yield. Broad template compatibility: Compatible with gDNA, FFPE DNA, cfDNA, amplicons, and other sample types. Robust performance across sample quality: Excellent performance on FFPE DNA with varying DIN values. Short library preparation time: Single library preparation completed within 90 minutes. |
| Package | 24 rxns DNA Dmage Repair Enzyme: 48 μL End Prep Buffer: 240 μL End Prep Enzyme: 120 μL Rapid Ligation Buffer 2: 600 μL Rapid DNA Ligase: 120 μL HiFi Amplification Mix: 600 μL PCR Primer Mix 3 for Illumina: 120 μL Control DNA (264 bp, 50 ng/μL): 10 μL |
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