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Sucrose Phosphorylase (SPL) from E. coli

Cat# DIA-437
Source E. coli
Form Freeze dried powder
Activity ≥ 50 U/mg
Isoelectric point 4.6
Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADPH per min at 25 °C and pH 6.8 under the conditions described below.
Storage Below –20 °C
Enzyme Commission Number EC 2.4.1.7
pH Stability 5.0–8.0
Michaelis Constant 3.9 × 10-2 M (Sucrose)
6.2 × 10-3 M (Phosphate)
Optimum pH 7.5
Optimum temperature 40 °C
Thermal stability Below 45 °C
Inhibitors Glucose, glucono-1,5-lactone
Applications For the determination of inorganic phosphate in clinical analysis.
Appearance White lyophilizate
Molecular Weight ca. 56 kDa (Gel filtration)
Notes Expiration date:
Liquid: stable at 37 °C for at least two weeks
Powder: stable at 30 °C for at least two weeks
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