| Cat# |
DIA-437 |
| Source |
E. coli |
| Form |
Freeze dried powder |
| Activity |
≥ 50 U/mg |
| Isoelectric point |
4.6 |
| Unit Definition |
One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADPH per min at 25 °C and pH 6.8 under the conditions described below. |
| Storage |
Below –20 °C |
| Enzyme Commission Number |
EC 2.4.1.7 |
| pH Stability |
5.0–8.0 |
| Michaelis Constant |
3.9 × 10-2 M (Sucrose) 6.2 × 10-3 M (Phosphate) |
| Optimum pH |
7.5 |
| Optimum temperature |
40 °C |
| Thermal stability |
Below 45 °C |
| Inhibitors |
Glucose, glucono-1,5-lactone |
| Applications |
For the determination of inorganic phosphate in clinical analysis. |
| Appearance |
White lyophilizate |
| Molecular Weight |
ca. 56 kDa (Gel filtration) |
| Notes |
Expiration date: Liquid: stable at 37 °C for at least two weeks Powder: stable at 30 °C for at least two weeks |
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