| Cat# | DIA-437 | 
                                        
                        | Source | E. coli | 
                                        
                        | Form | Freeze dried powder | 
                                        
                        | Activity | ≥ 50 U/mg | 
                                        
                        | Isoelectric point | 4.6 | 
                                        
                        | Unit Definition | One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADPH per min at 25 °C and pH 6.8 under the conditions described below. | 
                                        
                        | Storage | Below –20 °C | 
                                        
                        | Enzyme Commission Number | EC 2.4.1.7 | 
                                        
                        | pH Stability | 5.0–8.0 | 
                                        
                        | Michaelis Constant | 3.9 × 10-2 M (Sucrose) 6.2 × 10-3 M (Phosphate)
 | 
                                        
                        | Optimum pH | 7.5 | 
                                        
                        | Optimum temperature | 40 °C | 
                                        
                        | Thermal stability | Below 45 °C | 
                                        
                        | Inhibitors | Glucose, glucono-1,5-lactone | 
                                        
                        | Applications | For the determination of inorganic phosphate in clinical analysis. | 
                                        
                        | Appearance | White lyophilizate | 
                                        
                        | Molecular Weight | ca. 56 kDa (Gel filtration) | 
                                        
                        | Notes | Expiration date: Liquid: stable at 37 °C for at least two weeks
 Powder: stable at 30 °C for at least two weeks
 | 
                                        
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