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2x Frag/Prime Buffer V2

Cat# DIA-551
Description This RNA fragmentation reagent is designed for use in transcriptome library preparation workflows. Under elevated temperature conditions, RNA is randomly fragmented into short fragments through Mg2+-mediated hydrolysis, with fragment size controlled by incubation time. This reagent is intended as a replacement fragmentation buffer within compatible RNA-seq library preparation systems and supports flexible and controlled RNA fragmentation prior to downstream library construction.
Storage -30 °C to -15 °C
Applications Frag/Prime Buffer V2 is compatible with the Universal V10 RNA-seq Library Prep Kit for Illumina for library construction.
Compared with the Frag/Prime Buffer 2 supplied with the kit, this product is primarily suitable for:
(1) Fragmentation of input RNA (total RNA, purified mRNA, or rRNA-depleted RNA) dissolved in nuclease-free ddH2O;
(2) Input RNA samples dissolved in nuclease-free ddH2O that have been fragmented by other methods..
Product Overview Increased flexibility and monitoring in the library preparation workflow.
Ensures reliable library quality.
Package 960 μL
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