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HotStart Tth DNA Polymerase

Cat# DIA-458
Description Antibody-modified Tth DNA Polymerase. Antibody blocks polymerase activity at low temperatures, preventing non-specific amplification. More resistant to blood-derived inhibitors than Taq, with enhanced reverse transcription activity in the presence of Mn2+. Suitable for one-step RT-PCR. Glycerol-free, compatible with lyophilized formulations.
Antibody-modified Tth DNA Polymerase with hot-start function, inhibitor resistance, and enhanced reverse transcription activity for one-step RT-PCR.
Product Overview Product Components:
250 U:
10x PCR buffer: 500 μL
5x RT-PCR buffer (Mn2+ Free): 1 mL
HotStart Tth DNA Polymerase (5 U/μL): 50 μL
MnCl2 (25 mM): 300 μL
dNTPs (10 mM): 150 μL
1250 U:
10x PCR buffer: 2.5 mL
5x RT-PCR buffer (Mn2+ Free): 5 mL
HotStart Tth DNA Polymerase (5 U/μL): 250 μL
MnCl2 (25 mM): 1.5 mL
dNTPs (10 mM): 750 μL
5 KU:
HotStart Tth DNA Polymerase (5 U/μL): 1 mL
Product Features:
High efficiency: integrates reverse transcriptase and polymerase activity for one-step detection
High specificity: higher RT temperature (60–70 °C): enhances specificity
High tolerance: robust resistance to PCR inhibitors
Package 250 U/1250 U/5 KU
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