| Cat# | DIA-502 |
| Source | Bacillus stearothermophilus |
| Description | High purity recombinant α-glucosidase (Bacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.2.1.20 |
| Activity | ~ 35 U/mg (40 °C, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside) |
| CAS No. | 9001-42-7 |
| Optimum temperature | 60 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of α-D-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl α-D-glucopyranoside (5 mM), in sodium phosphate buffer (100 mM), pH 6.5 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | α-glucosidase; α-D-glucoside glucohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications in carbohydrate and biofuels research and analysis and analytical procedures. |
| Molecular Weight | 66000 Da |
| Concentration | ~ 1500 U/mL |
| Specificity | Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose. |
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