| Cat# | DIA-545 |
| Source | Aspergillus sp. |
| Description | High purity pectate lyase from Aspergillus sp. for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Solution |
| CAS No. | 9015-75-2 |
| Activity | ~ 200 U/mg (40 °C, pH 8.0 on polygalacturonic acid) |
| Unit Definition | One unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris HCl buffer (50 mM), pH 8.0 at 40 °C. |
| Storage | Below -10 °C |
| Synonyms | Pectate lyase; (1→4)-α-D-galacturonan lyase |
| Stability | > 1 year under recommended storage conditions |
| Optimum temperature | 55 °C |
| Buffer | 50% (v/v) glycerol |
| Applications | Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research. |
| Molecular Weight | 45000 Da |
| Concentration | ~ 1400 U/mL |
| Specificity | Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends. |
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