| Cat# | CAS-0906 |
| Source | E. coli |
| Description | Cytosine Base Editor (GMP grade) is a Cytosine Base Editor (CBE) fused from Cas9 Nickase, Deaminase, and UGI. Deaminates C to U at the target position (window 3-12). Creatively cages the deaminase, Active only upon sgRNA binding to the target, significantly reducing random binding, achieving near-zero off-target effects. Validated in primary NK and T cells. No DSB introduction ensures safety. |
| Storage | Dry ice shipping, -80 ± 10℃, avoid freeze-thaw |
| Purity | ≥ 88% (RP-HPLC）; ≥80% (SEC-HPLC) |
| Optimum pH | 8.0±0.5 |
| Buffer | 30 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 8.0 |
| Abbr | CBE |
| Applications | Single Base Editing; Induce stop codon to Knockout target gene; Mutate splice sites to Knockout target gene. |
| Appearance | Liquid |
| Molecular Weight | 210.14 kDa |
| Concentration | 9.0-11.0 mg/ml |
| Endotoxin Level | ≤10 EU/mg |
| Grade | GMP Grade |
| Download Datasheet: |  |
