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Maltose Phosphorylase (MPL) from E. coli

Cat# DIA-436
Source E. coli
Form Freeze dried powder
Activity ≥ 10 U/mg
Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of D-glucose per min at 30 °C and pH 7.0 under the conditions described below.
Storage Below –20 °C
Enzyme Commission Number EC 2.4.1.8
pH Stability 5.5–8.0
Michaelis Constant 1.9 × 10-3 M (Maltose)
3.4 × 10-3 M (Phosphate)
8.3 × 10-3 M (Arsenate)
Optimum pH 6.5–7.5
Optimum temperature 45–50°C
Thermal stability Below 55 °C
Inhibitors Hg2+, Ag+, Zn2+, Cu2+
Applications For the determination of α-amylase and inorganic phosphate in clinical analysis.
Appearance White lyophilizate
Molecular Weight ca. 220 kDa (Gel filtration)
Notes Expiration date:
Liquid: stable at 37 °C for at least one week
Powder: stable at 30 °C for at least four weeks
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