| Cat# | DIA-470 |
| Description | DNase I (Deoxyribonuclease I) is an endonuclease that randomly cleaves single-stranded or double-stranded DNA, generating oligonucleotides with 5′-phosphate termini. Its activity is highly dependent on Ca2+ and can be activated by Mg2+ or Mn2+. Under Mg2+ conditions, DNase I (lyophilized) can randomly cut dsDNA at any site; under Mn2+ conditions, it can cleave both strands at approximately the same site, producing blunt ends or sticky ends with 1–2 nucleotide overhangs. Glycerol-free, suitable for lyophilization. |
| Unit Definition | One unit (U) is defined as the amount of enzyme required to completely degrade 1 μg of pBR322 plasmid DNA in 10 minutes at 37 °C. |
| Storage | 2–8 °C, –20 °C ± 5 °C for long-term storage |
| Synonyms | Deoxyribonuclease I |
| Inhibitors | Metal chelators, 0.1% SDS, DTT, and mercaptoethanol |
| Contaminants | Free of E. coli DNA residues; free of endo-/exonuclease contamination |
| Applications | Preparation of DNA-free RNA samples; removal of genomic DNA contamination prior to RT-PCR; removal of DNA templates after in vitro transcription with T7, T3, or SP6 RNA polymerases; footprinting assays for DNA–protein interactions; used with DNA polymerase I for nick translation; DNA fragmentation to create random libraries in the presence of Mn<2+; positive control in TUNEL assays for apoptotic DNA fragmentation. |
| Product Overview | Product Components: 100 U: DNase I (Lyo): 100 U 25 mM EDTA: 500 μL 500 U: DNase I (Lyo): 500 U 25 mM EDTA: 2.5 mL Product Features: Quickly reconstitutes with RNase-Free Water No cold-chain transport required; stable at room temperature or 2–8 °C storage No additional reaction buffer required |
| Package | 100/500 U |
| Download Datasheet: |  |
