| Cat# | DIA-569 |
| Source | Escherichia coli |
| Species | Thermococcus kodakaraensis |
| Description | A high-fidelity thermostable DNA polymerase capable of amplifying DNA fragments up to 6 kb. It possesses 3′→5′ exonuclease proofreading activity, providing both high accuracy and high yield. Its extension rate and synthesis efficiency are approximately 5-fold and 10–15-fold higher than Pfu DNA polymerase, allowing rapid and reliable PCR amplification of target DNA. |
| Optimum pH | 6.5 |
| Optimum temperature | 75 °C |
| Unit Definition | One unit of enzyme activity is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75 °C. |
| Storage | -30 °C to -15 °C |
| Buffer | 50 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 1m MDTT |
| Applications | Construction of knockout targeting vectors, gene cloning, and synthesis; genomic DNA cloning. |
| Concentration | 1 U/µL |
| Purity | >90% (SDS-PAGE) |
| Package | 120 U/200 U 10× KOD Buffer (Mg2+): 1000 μL dNTP Mixture (2.5 mM): 350 μL KOD DNA polymerase (1 U/μL): 40 Ml |
| Download Datasheet: |  |
