| Cat# | DIA-190 |
| Abbr | Glucoamylase (Rhizopus sp.) |
| Source | Rhizopus sp. |
| Description | Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glycosidic bonds when the next bond in the sequence is 1,4. |
| Applications | This enzyme is useful for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis. |
| Appearance | White amorphous powder (salt-free), lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 3.2.1.3 |
| Activity | 30U/mg-solid or more |
| CAS No. | 9032-08-0 |
| pH Stability | pH 4.0-8.5 (25°C, 20hr) |
| Michaelis Constant | 11±1.1×10⁻⁴M (Maltose), 3.6±0.51×10⁻⁴M (Maltotriose), 2.5±0.33×10⁻⁴M (Maltotetraose), 1.6±0.02×10⁻⁴M (Maltopentaose) |
| Specificity | This enzyme completely hydrolyzes soluble starch, amylopectin, glycogen,α-orß-limit dextrin, amylose, maltooligosaccharides and panose. |
| Optimum pH | 4.5-5.0 |
| Optimum temperature | 60°C |
| Thermal stability | below 45°C (pH 5.5, 10min) |
| Stability | Stable at-20°C for at least 6 months |
| Synonyms | EC 3.2.1.3; glucoamylase; amyloglucosidase; gamma-amylase; lysosomal alpha-glucosidase; acid maltase; exo-1,4-alpha-glucosidase; glucose amylase; gamma-1,4-glucan glucohydrolase; acid maltase; 1,4-alpha-D-glucan glucohydrolase |
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