| Cat# | Kit-050 |
| Description | This kit is specifically designed for Illumina high-throughput sequencing platforms and enables efficient construction of sequencing-ready DNA libraries. Compared with conventional library preparation methods, it employs a transposase-based fragmentation strategy that integrates DNA fragmentation, end repair, and adapter ligation into a single enzymatic reaction. This approach significantly reduces required input DNA amounts and shortens library preparation time. The kit is available in formats optimized for 50 ng, 5 ng, and 1 ng input DNA, allowing flexible selection according to experimental requirements. All reagents are subjected to rigorous quality control and functional validation to ensure reliable, stable, and reproducible library construction. |
| Storage | -30 – -15 °C |
| Applications | Transposase-based library preparation; whole-genome sequencing; ATAC-seq; metagenomic sequencing. |
| Product Overview | Fast single-sample library preparation: Less than 90 minutes per sample. Single-tube enzymatic reaction: Fragmentation and adapter ligation occur simultaneously without mechanical shearing (ultrasound or nebulization). Optimized library amplification: Dedicated polymerase and buffer system ensure high efficiency and uniformity. Low input requirement: Supports starting DNA as low as 1 ng. Flexible kit specifications: Three kit versions based on input DNA amounts. High multiplexing capability: Provides 96 paired-end index combinations. |
| Package | 24 rxns Tagmet Enzyme Mix V50: 120 μL 5x Tagment Buffer L: 240 μL PCR Primer Mix: 120 μL Amplify Enzyme: 24 μL 5x Amplify Buffer: 240 μL Control DNA (Mouse Genomic DNA, 50 ng/μL): 10 mL |
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