| Cat# | Kit-059 |
| Description | Optimized for the Illumina platform, this kit supports input DNA from 100 pg to 4 μg. Comprehensive improvements to end-repair, ligation, and amplification modules enhance library conversion efficiency and amplification yield. It is suitable for various sample types, including PCR and PCR-free libraries, and is compatible with targeted capture workflows. All reagents undergo strict quality control and functional verification to ensure reproducible and reliable library construction. Compatible sample types include: genomic DNA (gDNA), cell-free DNA (cfDNA/ctDNA), FFPE DNA, ChIP DNA, and multiplexed amplicons. |
| Storage | -30 °C to -15 °C |
| Applications | Whole-genome sequencing (WGS); whole-exome sequencing (WES) or other targeted capture sequencing; amplicon sequencing; chromatin immunoprecipitation sequencing (ChIP-seq); metagenomic sequencing; methylation sequencing. |
| Product Overview | Shorter library preparation time: Single library preparation completed in as little as 75 minutes. Higher adapter ligation efficiency: Results in improved library conversion rates. Simplified and fast workflow: Pre-mixed reagents minimize hands-on steps and reduce operational errors. Wide input DNA compatibility: Supports starting DNA inputs from 100 pg to 4 μg. Broad sample compatibility: Suitable for fragmented DNA, cfDNA, FFPE DNA, ChIP DNA, and more. |
| Package | 24 rxns End Prep Mix 4: 360 μL Rapid Ligation Buffer 2: 600 μL Rapid DNA Ligase: 120 μL HiFi Amplification Mix: 600 μL PCR Primer Mix 3 for Illumina: 120 μL Control DNA (264 bp, 50 ng/μL): 10 μL |
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