| Cat# | DIA-503 |
| Source | Aspergillus niger |
| Description | High purity α-L-arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.2.1.55 |
| Activity | ~ 32 U/mg (40 °C, pH 4.0 on p-nitrophenyl-α-L-arabinofuranoside) ~ 12.6 U/mg (on sugar-beet arabinan) ~ 1.2 U/mg (on wheat arabinoxylan) |
| CAS No. | 9067-74-7 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Non-reducing end α-L-arabinofuranosidase; α-L-arabinofuranoside non-reducing end α-L-arabinofuranosidase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications in carbohydrate and biofuels research. |
| Molecular Weight | 62000 Da |
| Concentration | ~ 300 U/mL |
| Specificity | Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate. |
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