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Creatinine Amidohydrolase from Microorganism

Cat# DIA-427
Source Microorganism
Form Freeze dried powder
Activity ≥ 400.0 U/mg
Isoelectric point 5.3
Storage −25 °C to −15 °C
Enzyme Commission Number EC 3.5.2.10
Purity ≥ 90.0% (SDS-PAGE)
pH Stability pH 5.5–10.0 (25 °C, 16 h)
Michaelis Constant 5.0 × 10-2 M (Creatinine)
8.0 × 10-2 M (Creatine)
Optimum pH 7.0–8.0
Optimum temperature 65 °C
Thermal stability Below 65 °C (pH 8.0, 30 min)
Inhibitors Hg2+, Cu2+, Fe3+
Applications For enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.
Appearance White to faint yellow lyophilizate
Molecular Weight 29 kDa (SDS-PAGE)
Package 10/100/1000/10000 KU
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For research and industrial use only, not for personal medicinal use.

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