| Cat# |
DIA-510 |
| Source |
Hordeum Vulgare |
| Description |
High purity β-amylase (barley) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form |
Powder |
| ECNumber |
3.2.1.2 |
| Activity |
~ 600 U/mg (40 °C, pH 6.0 on soluble starch) |
| CAS No. |
9000-91-3 |
| Optimum temperature |
60 °C |
| Stability |
> 1 year under recommended storage conditions |
| Unit Definition |
One unit of β-amylase activity is defined as the amount of enzyme required to release one µmole of maltose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium phosphate buffer (200 mM), pH 6.0 at 40 °C. |
| Storage |
Below -10 °C |
| Synonyms |
β-amylase; 4-α-D-glucan maltohydrolase |
| Buffer |
BSA plus ammonium sulphate powder |
| Applications |
For use in AACC and ASBC α-amylase assay procedures. |
| Molecular Weight |
58300 Da |
| Specificity |
Hydrolysis of (1,4)-α-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains. |
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