| Cat# | DIA-586 |
| Source | Escherichia coli |
| Description | T4 polynucleotide kinase catalyzes the transfer of the γ-phosphate from ATP to the 5′-hydroxyl termini of DNA or RNA (single- or double-stranded), as well as to 3′-monophosphate nucleosides. In addition, it exhibits 3′-phosphatase activity, removing phosphate groups from 3′-phosphorylated oligonucleotides and deoxynucleoside mono- and diphosphates. |
| Unit Definition | One unit is defined as the amount of enzyme required to incorporate 1 nmol of [γ-32P] ATP into acid-insoluble material within 30 minutes at 37 °C. |
| Storage | -20 °C |
| Buffer | 50 mM Tris-HCl pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 μM ATP, 50% glycerol |
| Applications | Phosphorylation of DNA or RNA 5′ ends; end labeling of DNA or RNA; removal of 3′ phosphate groups; 5′ phosphorylation and 3′ dephosphorylation of insert fragments during library preparation. |
| Product Overview | 5′ phosphorylation of DNA or RNA ends for subsequent ligation reactions. End labeling of DNA or RNA for probe preparation or DNA sequencing applications. |
| Package | 10000 U T4 Polynucleotide Kinase (10 U/μL): 1 mL 10× T4 PNK Buffer (700 mM Tris-HCl pH 7.6, 100 mM MgCl2, 50 mM DTT): 2 mL |
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