| Cat# |
DIA-456 |
| Description |
This engineered Bst DNA polymerase retains 5′→3′ polymerase activity and strong strand displacement while lacking 5′→3′ exonuclease activity. Designed for high-efficiency, high-sensitivity isothermal amplification (e.g., LAMP). Includes accessory protein A, enhancing LAMP sensitivity by 10–100x. Integrates next-generation hot-start technology: enzyme activity is blocked below 50 °C and rapidly released above 50 °C, increasing specificity. Supports room-temperature reaction setup. Glycerol-free, suitable for lyophilization, with no excipients included. |
| Storage |
−30 °C to −15 °C |
| Applications |
LAMP/RT-LAMP isothermal amplification. |
| Product Overview |
Product Components: 1600 U: High-sensitivity Bst lll DNA Polymerase (Glycerol-free, 8 U/μL): 200 μL 10x HS lsothermalAmp Buffer: 1 mL MgSO4 (100 mM): 1 mL dNTP Mix (10 mM each): 1 mL 8000 U: High-sensitivity Bst lll DNA Polymerase (Glycerol-free, 8 U/μL): 1 mL 10x HS lsothermalAmp Buffer: 3 x 1 mL MgSO4(100 mM): 2 x 1 mL dNTP Mix (10 mM each): 4 mL Product Features: Strong amplification performance: high sensitivity and rapid speed Robust impurity tolerance: enables direct PCR from saliva, nasal swabs, and throat swabs Supports room temperature system setup |
| Package |
1600/8000 U |
| Download Datasheet: |
 |