| Cat# | DIA-513 |
| Source | Agrobacterium sp. |
| Description | High purity β-glucosidase (Agrobacterium sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| Activity | ~ 120 U/mg (40 °C, pH 6.5 on p-nitrophenyl β-glucoside) |
| CAS No. | 9001-22-3 |
| Optimum temperature | 50 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of β-glucosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-glucopyranoside (10 mM) in sodium maleate buffer (50 mM), pH 6.5 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | β-glucosidase; β-D-glucoside glucohydrolase |
| Buffer | 3.2 M ammonium sulphate (stabilized with BSA) |
| Applications | Applications established in analysis and research within the food and feed, carbohydrate and biofuels industries. |
| Molecular Weight | 52200 Da |
| Concentration | ~ 400 U/mL |
| Specificity | Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose. |
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